How do you explain a very high positive ds-DNA antibody by Elisa but negative by IFA?

Different readouts using ELISA platforms vs Cell/organelle-based assay systems may arise due to target epitopes being more biophysically accessible in an ELISA platform than they are in whole cell/organelle preparations. As such, as outlined by Dr. @Dr. First Last, it is probably prudent to use a va...

Dr. @Elena Cucurull: I have a similar patient. Her ELISA is > 300 while her IFA is negative. Her ELISA has never fluctuated with disease activity. She ended up getting MALT and lupus myocarditis, making me wonder if the very high anti-dsDNA may have been pathogenic in some way (or at least reflect h...

Very helpful! Thank you.

J Lavery MD